Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene

Yousif Elhassaneen; Sherif Ragab; Riham Badawy

doi:doi:10.11648/j.wjph.20170201.15

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Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene

Yousif Elhassaneen, Sherif Ragab, Riham Badawy

Published in World Journal of Public Health (Volume 2, Issue 1)

Received: 4 November 2016 Accepted: 24 November 2016 Published: 7 January 2017

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Abstract

In this study, we evaluate the antioxidant effect and total phenolics content of four selected plant parts [prickly pear peel (PPP), turmeric rhizomes (TR), red onion skin (ROS) and eggplant peel (EP)] methanolic extracts and their potential role in improving the liver disorders induced by an ubiquitous food pollutant i.e. benzo(a)pyrene (BP). The selected plant parts methanolic extracts showed considerable differences in antioxidant activity (AA=57.51 to 90.73 %) and total phenolics (11.29 to 211.45 mg GAE. g ^-1). When all selected plant parts methanolic extracts were included in the statistical analysis, there was a positive and highly significant (r²=0.597-0.919, p≤ 0.05) relationship between total phenolics and antioxidant activity. Also, the antioxidant potential of those methanolic extracts against BP was also studied in vitro in a model using Catfish liver cells in homogenate culture. BP induced many metabolic disorders and oxidative stress in fish liver homogenate namely a significant decrease in reduced glutathione (GSH) and albumin (Alb) content, and increase in malondialdehyde (MDA) formation after 24 hours of culture. Co-treatment of liver homogenate with BP and the tested selected plant parts extracts as well as their mixture by concentration 0.75% exhibited some protection effects through decreasing the rates of all those metabolic disorders and oxidative stress. That decreasing rates in different adverse effects was depending on the type of the plant parts applied. The highest therapeutic effect was recorded for the mixture of the selected plant parts extracts (PPP+TR+ROS+EP by equal amounts) followed by ROS, EP, TR and PPP, respectively. This study indicates that the compounds present in those selected extracts contain interesting bioactivities which improve many adverse effects i.e. metabolic disorders and oxidative stress in liver cells induced by BP.

Published in	World Journal of Public Health (Volume 2, Issue 1)
DOI	10.11648/j.wjph.20170201.15
Page(s)	38-50
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cat Liver Homogenate, Total Phenolics, Metabolic Disorders, Oxidative Stress, Onion Skin, Eggplant Peel, Prickly Pear Peel

References

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Yousif Elhassaneen, Sherif Ragab, Riham Badawy. (2017). Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene. World Journal of Public Health, 2(1), 38-50. https://doi.org/10.11648/j.wjph.20170201.15

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Yousif Elhassaneen; Sherif Ragab; Riham Badawy. Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene. World J. Public Health 2017, 2(1), 38-50. doi: 10.11648/j.wjph.20170201.15

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AMA Style

Yousif Elhassaneen, Sherif Ragab, Riham Badawy. Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene. World J Public Health. 2017;2(1):38-50. doi: 10.11648/j.wjph.20170201.15

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@article{10.11648/j.wjph.20170201.15,
  author = {Yousif Elhassaneen and Sherif Ragab and Riham Badawy},
  title = {Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene},
  journal = {World Journal of Public Health},
  volume = {2},
  number = {1},
  pages = {38-50},
  doi = {10.11648/j.wjph.20170201.15},
  url = {https://doi.org/10.11648/j.wjph.20170201.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20170201.15},
  abstract = {In this study, we evaluate the antioxidant effect and total phenolics content of four selected plant parts [prickly pear peel (PPP), turmeric rhizomes (TR), red onion skin (ROS) and eggplant peel (EP)] methanolic extracts and their potential role in improving the liver disorders induced by an ubiquitous food pollutant i.e. benzo(a)pyrene (BP). The selected plant parts methanolic extracts showed considerable differences in antioxidant activity (AA=57.51 to 90.73 %) and total phenolics (11.29 to 211.45 mg GAE. g -1). When all selected plant parts methanolic extracts were included in the statistical analysis, there was a positive and highly significant (r2=0.597-0.919, p≤ 0.05) relationship between total phenolics and antioxidant activity. Also, the antioxidant potential of those methanolic extracts against BP was also studied in vitro in a model using Catfish liver cells in homogenate culture. BP induced many metabolic disorders and oxidative stress in fish liver homogenate namely a significant decrease in reduced glutathione (GSH) and albumin (Alb) content, and increase in malondialdehyde (MDA) formation after 24 hours of culture. Co-treatment of liver homogenate with BP and the tested selected plant parts extracts as well as their mixture by concentration 0.75% exhibited some protection effects through decreasing the rates of all those metabolic disorders and oxidative stress. That decreasing rates in different adverse effects was depending on the type of the plant parts applied. The highest therapeutic effect was recorded for the mixture of the selected plant parts extracts (PPP+TR+ROS+EP by equal amounts) followed by ROS, EP, TR and PPP, respectively. This study indicates that the compounds present in those selected extracts contain interesting bioactivities which improve many adverse effects i.e. metabolic disorders and oxidative stress in liver cells induced by BP.},
 year = {2017}
}

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TY  - JOUR
T1  - Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene
AU  - Yousif Elhassaneen
AU  - Sherif Ragab
AU  - Riham Badawy
Y1  - 2017/01/07
PY  - 2017
N1  - https://doi.org/10.11648/j.wjph.20170201.15
DO  - 10.11648/j.wjph.20170201.15
T2  - World Journal of Public Health
JF  - World Journal of Public Health
JO  - World Journal of Public Health
SP  - 38
EP  - 50
PB  - Science Publishing Group
SN  - 2637-6059
UR  - https://doi.org/10.11648/j.wjph.20170201.15
AB  - In this study, we evaluate the antioxidant effect and total phenolics content of four selected plant parts [prickly pear peel (PPP), turmeric rhizomes (TR), red onion skin (ROS) and eggplant peel (EP)] methanolic extracts and their potential role in improving the liver disorders induced by an ubiquitous food pollutant i.e. benzo(a)pyrene (BP). The selected plant parts methanolic extracts showed considerable differences in antioxidant activity (AA=57.51 to 90.73 %) and total phenolics (11.29 to 211.45 mg GAE. g -1). When all selected plant parts methanolic extracts were included in the statistical analysis, there was a positive and highly significant (r2=0.597-0.919, p≤ 0.05) relationship between total phenolics and antioxidant activity. Also, the antioxidant potential of those methanolic extracts against BP was also studied in vitro in a model using Catfish liver cells in homogenate culture. BP induced many metabolic disorders and oxidative stress in fish liver homogenate namely a significant decrease in reduced glutathione (GSH) and albumin (Alb) content, and increase in malondialdehyde (MDA) formation after 24 hours of culture. Co-treatment of liver homogenate with BP and the tested selected plant parts extracts as well as their mixture by concentration 0.75% exhibited some protection effects through decreasing the rates of all those metabolic disorders and oxidative stress. That decreasing rates in different adverse effects was depending on the type of the plant parts applied. The highest therapeutic effect was recorded for the mixture of the selected plant parts extracts (PPP+TR+ROS+EP by equal amounts) followed by ROS, EP, TR and PPP, respectively. This study indicates that the compounds present in those selected extracts contain interesting bioactivities which improve many adverse effects i.e. metabolic disorders and oxidative stress in liver cells induced by BP.
VL  - 2
IS  - 1
ER  -

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Author Information

Yousif Elhassaneen

Nutrition and Food Science Department, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt
Sherif Ragab

Nutrition and Food Science Department, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt
Riham Badawy

Nutrition and Food Science Department, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt

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Yousif Elhassaneen, Sherif Ragab, Riham Badawy. (2017). Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene. World Journal of Public Health, 2(1), 38-50. https://doi.org/10.11648/j.wjph.20170201.15

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Yousif Elhassaneen; Sherif Ragab; Riham Badawy. Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene. World J. Public Health 2017, 2(1), 38-50. doi: 10.11648/j.wjph.20170201.15

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AMA Style

Yousif Elhassaneen, Sherif Ragab, Riham Badawy. Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene. World J Public Health. 2017;2(1):38-50. doi: 10.11648/j.wjph.20170201.15

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@article{10.11648/j.wjph.20170201.15,
  author = {Yousif Elhassaneen and Sherif Ragab and Riham Badawy},
  title = {Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene},
  journal = {World Journal of Public Health},
  volume = {2},
  number = {1},
  pages = {38-50},
  doi = {10.11648/j.wjph.20170201.15},
  url = {https://doi.org/10.11648/j.wjph.20170201.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20170201.15},
  abstract = {In this study, we evaluate the antioxidant effect and total phenolics content of four selected plant parts [prickly pear peel (PPP), turmeric rhizomes (TR), red onion skin (ROS) and eggplant peel (EP)] methanolic extracts and their potential role in improving the liver disorders induced by an ubiquitous food pollutant i.e. benzo(a)pyrene (BP). The selected plant parts methanolic extracts showed considerable differences in antioxidant activity (AA=57.51 to 90.73 %) and total phenolics (11.29 to 211.45 mg GAE. g -1). When all selected plant parts methanolic extracts were included in the statistical analysis, there was a positive and highly significant (r2=0.597-0.919, p≤ 0.05) relationship between total phenolics and antioxidant activity. Also, the antioxidant potential of those methanolic extracts against BP was also studied in vitro in a model using Catfish liver cells in homogenate culture. BP induced many metabolic disorders and oxidative stress in fish liver homogenate namely a significant decrease in reduced glutathione (GSH) and albumin (Alb) content, and increase in malondialdehyde (MDA) formation after 24 hours of culture. Co-treatment of liver homogenate with BP and the tested selected plant parts extracts as well as their mixture by concentration 0.75% exhibited some protection effects through decreasing the rates of all those metabolic disorders and oxidative stress. That decreasing rates in different adverse effects was depending on the type of the plant parts applied. The highest therapeutic effect was recorded for the mixture of the selected plant parts extracts (PPP+TR+ROS+EP by equal amounts) followed by ROS, EP, TR and PPP, respectively. This study indicates that the compounds present in those selected extracts contain interesting bioactivities which improve many adverse effects i.e. metabolic disorders and oxidative stress in liver cells induced by BP.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene
AU  - Yousif Elhassaneen
AU  - Sherif Ragab
AU  - Riham Badawy
Y1  - 2017/01/07
PY  - 2017
N1  - https://doi.org/10.11648/j.wjph.20170201.15
DO  - 10.11648/j.wjph.20170201.15
T2  - World Journal of Public Health
JF  - World Journal of Public Health
JO  - World Journal of Public Health
SP  - 38
EP  - 50
PB  - Science Publishing Group
SN  - 2637-6059
UR  - https://doi.org/10.11648/j.wjph.20170201.15
AB  - In this study, we evaluate the antioxidant effect and total phenolics content of four selected plant parts [prickly pear peel (PPP), turmeric rhizomes (TR), red onion skin (ROS) and eggplant peel (EP)] methanolic extracts and their potential role in improving the liver disorders induced by an ubiquitous food pollutant i.e. benzo(a)pyrene (BP). The selected plant parts methanolic extracts showed considerable differences in antioxidant activity (AA=57.51 to 90.73 %) and total phenolics (11.29 to 211.45 mg GAE. g -1). When all selected plant parts methanolic extracts were included in the statistical analysis, there was a positive and highly significant (r2=0.597-0.919, p≤ 0.05) relationship between total phenolics and antioxidant activity. Also, the antioxidant potential of those methanolic extracts against BP was also studied in vitro in a model using Catfish liver cells in homogenate culture. BP induced many metabolic disorders and oxidative stress in fish liver homogenate namely a significant decrease in reduced glutathione (GSH) and albumin (Alb) content, and increase in malondialdehyde (MDA) formation after 24 hours of culture. Co-treatment of liver homogenate with BP and the tested selected plant parts extracts as well as their mixture by concentration 0.75% exhibited some protection effects through decreasing the rates of all those metabolic disorders and oxidative stress. That decreasing rates in different adverse effects was depending on the type of the plant parts applied. The highest therapeutic effect was recorded for the mixture of the selected plant parts extracts (PPP+TR+ROS+EP by equal amounts) followed by ROS, EP, TR and PPP, respectively. This study indicates that the compounds present in those selected extracts contain interesting bioactivities which improve many adverse effects i.e. metabolic disorders and oxidative stress in liver cells induced by BP.
VL  - 2
IS  - 1
ER  -

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